Identification of Pathogenic Bacteria from Kilishi Meat Sold in Ka-shere Municipal: Repercussions for Public Health and Food Security
DOI:
https://doi.org/10.59652/aim.v3i2.585Keywords:
Microbial contamination, Kilishi, pathogenic bacteria, Gombe State, food securityAbstract
Kilishi, a popular dried meat snack predominantly consumed in northern Nigeria, is often accompanying with lacking hygienic practices during its preparation, management, and storage. This study aimed to identify the pathogenic bacteria in Kilishi sold in the Kashere municipal, Gombe State, Nigeria. A total of 30 Kilishi samples were collected from three different locations: Bakin Tasha (Spot 1), Bakin Cross (Spot 2), and Bubabani (Spot 3). The samples were examined through standard microbiological methods, including bacteriological analysis, Gram staining, and biochemical tests. The results disclosed significant bacterial infection across all samples, with Spot 3 unveiling the highest coliform count of 2.9 × 10¹º CFU/mL, followed by Spot 2 (2.5 × 10⁹ CFU/mL) and Spot 1 (1.7 × 10⁹ CFU/mL). Six bacterial species were recognized, with Bacillus species being the most predominant (28.1%), followed by Escherichia coli (23.4%), Staphylococcus species (17.2%), Pseudomonas species (12.5%), Proteus species (4.7%), and Salmonella species (14.1%). The presence of these pathogenic bacteria highlights a serious public health risk. The study underscores the need for improved hygienic practices during the manufacture, management, and storage of Kilishi to reduce microbial contamination and ensure user safety.
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Gahamanyi N, Mboera LEG, Matee MI, Mutangana D, Komba EVG. Prevalence, Risk Factors, and Antimicrobial Resistance Profiles of Thermophilic Campylobacter Species in Humans and Animals in Sub-Saharan Africa: A Systematic Review. Int J Mi-crobiol. 2020;2020:2092478.
Frazier WC, Westhoff DC. Food microbiology. 4th ed. New Delhi: Tata McGraw Hill; 2008. 539 p.
Inusa SK, Muhammad BF. Evaluation of chemical properties of Kilishi prepared from fermented cattle and camel beef in semi-arid Nigeria. Nigerian Journal of Animal Production. 2021;48(6):88–97.
Zhu Y, Wang W, Li M, Zhang J, Ji L, Zhao Z, et al. Microbial diversity of meat products under spoilage and its controlling ap-proaches. Front Nutr. 2022 Dec 1;9:1078201.
Dahiru A. T, K MA. Bacteriological Quality Assessment of Kilishi Produced in Kunchi Local Government Area, Kano State, Nigeria. UMYU Journal of Microbiology Research (UJMR). 2019 Jun 30;4(1):12–8.
Hazards (BIOHAZ) EP on B. Growth of spoilage bacteria during storage and transport of meat. EFSA Journal. 2016;14(6):e04523.
Bersisa A, Tulu D, Negera C. Investigation of Bacteriological Quality of Meat from Abattoir and Butcher Shops in Bishoftu, Central Ethiopia. Int J Microbiol. 2019;2019:6416803.
Borch E, Kant-Muermans ML, Blixt Y. Bacterial spoilage of meat and cured meat products. Int J Food Microbiol. 1996 Nov;33(1):103–20.
Kenaw Z, Ejeso A, Deresse D, Olkeba BK. Microbial Contamination and Meat Handling Practices in Hawassa City Butcher Shops, Ethiopia. Environ Health Insights. 2024 Oct 28;18:11786302241293289.
Kim HJ, Kim HJ, Hong H, Jo C. Meat-derived broth as a novel culture medium for metabolomic study of bacteria in meat. LWT. 2024 Jun 1;201:116254.
Teshome G, Assefa Z, Keba A. Assessment of microbial quality status of raw beef around Addis Ababa city, Ethiopia. AJFS. 2020 Aug 31;14(7):209–14.
Cheesbrough M. District Laboratory Practice in Tropical Countries [Internet]. 2nd ed. Cambridge: Cambridge University Press; 2006 [cited 2025 Jun 29]. Available from: https://www.cambridge.org/core/books/district-laboratory-practice-in-tropical-countries/6F6FE267756B004A4826209C692BD5B3
Jabaka RD, Ododife Q, Daniel AD, Nuhu UD, Doro EJ, Jibo RJ, et al. Assessment of Bacteria and Parasite Contamination of Dried Sliced Beef (Kilishi) Sold within Birnin Kebbi Metropolis, Kebbi State, Northern Nigeria. South Asian Journal of Research in Microbiology. 2020;58–66.
Abolagba D. A Comparative Analysis of the Microbial Load of Smoke-Dried Fishes (Ethmalosa Fimbriata and Pseudotolithus Elongatus) Sold in Oba and Koko Markets in Edo and Delta States, Nigeria at Different Seasons. 2011;
Olayinka EM, Temitope OA, Innocent DC. Evaluation of microbial hazards associated with the processing of Suya (a grilled meat product). SRE. 2008 Dec 31;3(12):621–6.
Drzewiecka D. Significance and Roles of Proteus spp. Bacteria in Natural Environments. Microb Ecol. 2016;72(4):741–58.
Myemba DT, Bwire GM, Sangeda RZ. Microbiological Quality of Selected Local and Imported Non-Sterile Pharmaceutical Products in Dar es Salaam, Tanzania. Infect Drug Resist. 2022 Apr 21;15:2021–34.
Food I of M (US) and NRC (US) C on the R of the U of SC and PS for S. International Microbiological Criteria. In: Scientific Criteria to Ensure Safe Food [Internet]. National Academies Press (US); 2003 [cited 2025 Jun 29]. Available from: https://www.ncbi.nlm.nih.gov/books/NBK221566/
Daminabo, V., Isu,, N. R., & Agarry. Antibiotic resistance profile of Enterococcal isolated from dried beef crackers (kilishi). Re-searchGate. 2013;1(5):35–9.
U AN, K Y, D D, A KF, B BM, Y MF, et al. The Bacterial Contaminants Associated with Kilishi Sold within Minna Metropolis, Nigeria. Journal of Advance Research in Pharmacy and Biological Science (ISSN 2208-2360). 2016 Apr 30;2(4):06–9.
O OM, O ME, P UU, T JJ, B G, A AM. Microflora analysis of sellected meat and meat products from Calabar, Cross River State-Nigeria | Abstract. [cited 2025 Jun 29]; Available from: https://www.scholarsresearchlibrary.com/abstract/microflora-analysis-of-sellected-meat-and-meat-products-from-calabar-cross-river-statenigeria-1117.html
Abdullahi, Nura, Ariahu, C C, Abu, O J. Effects of Chemical Hurdles and Packaging Materials on Microbial Load and Bacterial Distribution in Kilishi under Ambient Storage. International Journal of Engineering Research & Technology [Internet]. 2016 Aug 20 [cited 2025 Jun 29];5(8). Available from: https://www.ijert.org/research/effects-of-chemical-hurdles-and-packaging-materials-on-microbial-load-and-bacterial-distribution-in-kilishi-under-ambient-storage-IJERTV5IS080256.pdf, https://www.ijert.org/effects-of-chemical-hurdles-and-packaging-materials-on-microbial-load-and-bacterial-distribution-in-kilishi-under-ambient-storage
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