Effects of Administration of Bouillon Cubes on Insulin Resistance, Lipid Profile and Renal Function Parameters in Female Albino Rats
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Abstract
Aim: To evaluate the effects of administration of bouillon cubes on insulin resistance, lipid profile and renal function parameters in female albino rats.
Methodology: A total of thirty-five (35) female albino rats, weighing between 120 and 150 grams, were used for the study. The bouillon cubes, Star Maggi and Knorr were administered daily to the rats, using an oral gavage tube for 90days. Fasting plasma glucose (FPG) was determined using the Glucose oxidase method. Fasting plasma insulin (FPI) and Cystatin C levels were quantitatively determined by a rat-specific sandwich-enzyme linked immunosorbent assay (ELISA) method. Insulin resistance was determined using the homeostatic model assessment for insulin resistance (HOMA-IR) method. The electrolytes, sodium (Na+) and potassium (K+) and were determined using ion selective electrode method. Urea was determined using Urease-bertholet method. Creatinine was determined using the Jaffe-Slot method. Total Cholesterol (TC), Triglyceride (TG) and High-Density Lipoprotein Cholesterol (HDL-C) were determined by enzymatic methods. Low Density Lipoprotein Cholesterol (LDL-C) was calculated from the Friedewald’s equation. Kidney sections were stained using haematoxylin and eosin (H&E) staining technique. Quantitative analysis of monosodium glutamate (MSG) content of the bouillon cubes was analyzed using ultraviolet (UV) spectroscopy while the sodium content was analyzed using atomic absorption spectrophotometry according to the method of the American Public Health Association.
Results: There were no significant differences (P>.05) in FPG, FPI and HOMA-IR in all the treatment groups. The mean cystatin C value in group E (High Dose Knorr) was significantly higher (P <.05) than the negative control and all other treatment groups. The results also show the mean sodium values in groups D (High Dose Maggi) and E (High Dose Knorr) were significantly lower (P <.05) when compared to the negative control. There were no significant differences (P >.05) in TC and HDL-C levels in the negative control, compared to the treatment groups. There were no significant differences (P >.05) in TG levels, except for group B (Low Dose Maggi) which significantly lower (P <.05) than the negative control. Also, there were no significant differences (P >.05) in LDL-C levels, except for group B (Low Dose Maggi) which significantly higher (P <.05) than the negative control. Histologic analysis of the kidneys of the treated groups showed histological changes in the architecture of the tissues indicating tissue distortion, acute tissue damage, glomerular nephritis and distorted capillaries and degeneration compared to the negative control group which showed no tissue distortion.
Conclusion: Chronic exposure to bouillon cubes did not impact fasting plasma glucose, insulin and insulin resistance in the treated rats. Chronic administration of Knorr cubes impacted the integrity of the kidney as levels of cystatin C and sodium were altered in the albino rats. Histoarchitecture of the kidneys of the treated rats showed histological changes indicating tissue distortion, acute tissue damage, glomerular nephritis and distorted capillaries. Lipid profile/metabolism was relatively not affected by the administration of bouillon cubes.
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References
Nwajei, J. C., Onuoha, S. C., & Essien, E. B. (2015). Effects of oral administration of selected food seasonings consumed in Nigeria on some sex hormones of Wistar albino rats. IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB), 1(5), 15-21.
Nestle Nigeria. (2011). Annual Report of Nestle Nigeria, 2011. Retrieved from https://www.nestle-cwa.com/sites/g/files/pydnoa346/files/asset-library/documents/nestle_2011_annual_report_.pdf
Inuwa, H. M., Aina, V. O., Gabi, B., Aimola, I., & Ja’afuru, L. (2011). Determination of nephrotoxicity and hepatotoxicity of monosodium glutamate (MSG) consumption. British Journal of Pharmacology and Toxicology, 2(3), 148-153.
Umedum, N. L., Udeozo, I. P., Muoneme, O., Okoye, N., & Iloamaka, I. (2013). Proximate analysis and mineral content of three commonly used seasonings in Nigeria. Journal of Environmental Science, Toxicology and Food Technology, 5, 11-14.
Tandel, K. R. (2011). Sugar substitutes: Health controversy over perceived benefits. Journal of Pharmacological Pharmacotherapy, 2(4), 236-243.
Organisation for Economic Co-operation and Development. (2001). Guidance document on acute oral toxicity testing: Environmental health and safety monograph series on testing and assessment No. 24. Retrieved from https://ntp.niehs.nih.gov/iccvam/suppdocs/feddocs/oecd/oecd-gd24.pdf
Erhirhie, E. O., Ekene, N. E., & Ajaghaku, D. L. (2014). Guidelines on dosage calculation and stock solution preparation in experimental animals’ studies. Journal of Natural Sciences Research, 4(18), 100–105.
Barham, D., & Trinder, P. (1972). An improved colour reagent for the determination of blood glucose by the oxidase system. Analyst, 97(151), 142-145.
Engvall, E., & Perlmann, P. (1972). Enzyme-linked immunosorbent assay, ELISA. The Journal of Immunology, 109(1), 129-135.
Matthews, D. R., Hosker, J. P., Rudenski, A. S., Naylor, B. A., Treacher, D. F., & Turner, R. C. (1985). Homeostasis model assessment: Insulin resistance and beta-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia, 28(7), 412-419.
Buck, R., & Lindner, E. (1994). Recommendations for nomenclature of ion selective electrodes (IUPAC Recommendations 1994). Pure and Applied Chemistry, 66(12), 2527-2536. Retrieved from https://doi.org/10.1351/pac199466122527
Bretaudiere, J. P., Phung, H. T., & Bailly, M. (1976). Direct enzymatic determination of urea in plasma and urine with a centrifugal analyzer. Clinical Chemistry, 22(10), 1614-1617.
Slot, C. (1965). Plasma creatinine determination. A new and specific Jaffe reaction method. Scandinavian Journal of Clinical and Laboratory Investigation, 17(4), 381-387. DOI: 10.3109/00365516509077065
Allain, C. C., Pooon, L. S., Cicely, S. G. C., Richmond, W., & Fu, P. C. (1974). Enzymatic determinants of total serum cholesterol. Journal of Clinical Chemistry, 20(4), 470–475.
Tietz, N. W. (1990). A Clinical Guide to Laboratory Tests (2nd Ed.). Philadelphia: WB. Sanders.
Lopes-Virella, M. F., Stone, P., & Colwell, J. (1977). Cholesterol determination in high-density lipoproteins separated by three different methods. Clinical Chemistry, 28, 882–884.
Friedewald, W. T., Levy, R. I., & Fredickson, D. S. (1972). Estimation of the concentration of LDL cholesterol in plasma without the use of the preparative ultra-centrifugation. Journal of Clinical Chemistry, 18, 499–502.
American Public Health Association. (1995). Standard methods for the determination of sodium using Agilent FS240AA Atomic Absorption Spectrophotometer. American Journal of Public Health, 9th edition. Byrd Prepess Springfield, Washington DC.
World Health Organization (WHO). (2013). Global Action Plan for the Prevention and control of Noncommunicable Diseases 2013-2020.
Apkanyung, E. O. (2005). Proximate and mineral composition of bouillon cubes produced in Nigeria. Pakistan Journal of Nutrition, 4(5), 327-329.
Alonge, P. O., Idemudia, O. S., & Odokuma-Alonge, O. (2019). Direct assay of monosodium glutamate in multi-sourced bouillon cubes by first derivative potentiometric titration. Journal of Applied Sciences and Environmental Management, 23(2), 299-304.
Briggs, O. N., Elechi-Amadi, K. N., Ohaka, J. C., Nwachuku, E. O., & Bartimaeus, E. S. (2021). Effects of metformin in combination with a herbal capsule (Glucoblock) on insulin resistance and oxidative stress index in type 2 diabetic rats. Journal of Complementary and Alternative Medical Research, 14(3), 18-25.
Tchaou, M. N., Lamboni, C., Eklu-Gadegbeku, K., Abalokoka, E., & Aklikokou, K. A. (2013). Effects of Food Flavour Enhancer (Monosodium Glutamate and Maggi Poulet) Supplementation on Glucose Tolerance in Sprague Dawley Rat. International Journal of Bio-logical and Chemical Science, 7(1), 161 – 171.
Husarova, V., & Ostatnikova, D. (2013). Monosodium glutamate toxic effects and their implications for human intake: a review. The Journal of Medical Research, 1–12. doi: 10.5171/2013.608765.
Zanfirescu, A., Ungurianu, A., Tsatsakis, A. M., Nițulescu, G. M., Kouretas, D., Veskoukis, A., Tsoukalas, D., Engin, A. B., Aschner, M., & Margină, D. (2019). A review of the alleged health hazards of monosodium glutamate. Comprehensive Reviews in Food Science and Food Safety, 18(4), 1111-1134. doi:10.1111/1541-4337.12448.
Paul, M. V., Abhilash, M., Varghese, M. V., Alex, M., & Nair, R. H. (2012). Protective effects of alpha-tocopherol against oxidative stress related to nephrotoxicity by monosodium glutamate in rats. Toxicology Mechanisms and Methods, 22(8), 625-630.
Tawfik, M. S., & Al-Badr, N. (2012). Adverse effects of monosodium glutamate on liver and kidney functions in adult rats and potential protective effect of vitamins C and E. Food and Nutrition Sciences, 3(5), 651-659.
Baky, N. A., Mohamed, A. M., & Faddah, L. M. (2009). Protective effect of N-acetyl cysteine and/or pro vitamin A against monosodium glutamate-induced cardiopathy in rats. Journal of Pharmacology and Toxicology, 4(5), 178–193.
Nahed, A. H., Amani, A. A., & Mamdouh, M. E. (2017). Effect of sesame on liver enzymes and lipid profile in rats exposed to oxidative stress induced by monosodium glutamate. Journal of American Science, 13(1), 71-78. doi:10.7537/marsjas130117.10.
Oodee, B. B., Briggs, O. N., Ben-Chioma, A. E., & Nwachuku, E. O. (2023). Effects of chronic administration of some bouillon cubes on oxidative stress and liver function markers in female albino rats. World Journal of Biology Pharmacy and Health Sciences, 16(1), 52-59.
Slima, S. R., & Ragab, R. (2023). Protective effect of curcumin against monosodium glutamate-induced oxidative renal damage: biochemical and histopathological study. Ain Shams Journal of Forensic Medicine and Clinical Toxicology, 40, 68-75.
Nnadozie, J. O., Chijioke, U. O., Okafor, O. C., Olusina, B. D., Oli, A. N., Nwonu, P. C., Mbagwu, H. O., & Chijioke, C. P. (2019). Chronic toxicity of low dose monosodium glutamate in albino Wistar rats. BMC Research Notes, 12(593), 1-7. https://doi.org/10.1186/s13104-019-4611-7